Introduction
Lactobacillus plantarum JLA-9, isolated from Suan-Tsai, a traditional Chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16S rDNA sequence analysis. A new bacteriocin, designated plantaricin JLA-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatography. The molecular mass of plantaricin JLA-9 was shown to be 1044 Da by MALDI-TOF-MS analyses. The amino acid sequence of plantaricin JLA-9 was predicted to be FWQKMSFA by MALDI-TOF-MS/MS, which was confirmed by Edman degradation. This bacteriocin exhibited broad-spectrum antibacterial activity against Gram-positive and Gram-negative bacteria, especially Bacillus spp., high thermal stability (20 min, 121 °C), and narrow pH stability (pH 2.0–7.0). It was sensitive to α-chymotrypsin, pepsin, alkaline protease, and papain. The mode of action of this bacteriocin responsible for outgrowth inhibition of Bacillus cereus spores was studied. Plantaricin JLA-9 had no detectable effects on germination initiation over 1 h on monitoring the hydration, heat resistance, and 2,6-pyridinedicarboxylic acid (DPA) release of spores. Rather, germination initiation is a prerequisite for the action of plantaricin JLA-9. Plantaricin JLA-9 inhibited growth by preventing the establishment of oxidative metabolism and disrupting membrane integrity in germinating spores within 2 h. The results suggest that plantaricin JLA-9 has potential applications in the control of Bacillus spp. in the food industry.
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Storage
Keep at 5-15DC and dry out of sunlight, keep away from children.
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| LIST OF STRAINS |
| Probiotics | Strain Number | Type(CFU/g) | |
| Lactiplantibacillus plantarum | Lp3a | 5-500B | |
| Lacticaseibacillus rhamnosus | LR05 | 5-400B | |
| Lacticaseibacillus casei | LC11 | 5-400B | |
| Lacticaseibacillus paracasei | LPC45 | 5-400B | |
| Lactobacillus fermentum | LF021 | 5-300B | |
| Lactobacillus salivarius | LS01 | 5-100B | |
| Lactobacillus reuteri | LR06 | 5-200B | |
| Lactobacillus acidophi/us | LA16 | 5-200B | |
| Lactobacillus helveticus | LH030/LZ-R-5 | 5-200B | |
| Lactobacillus crispatus | LCP051 | 5-100B | |
| Lactobacillus gasseri | LG021/LG019 | 5-200B | |
| Lactobacillus johnsonii | LJ10 | 5-100B | |
| Lactobacillus delbrueckii subsp. bulgaricus | LB45 | 5-20B | |
| Bifidobacterium animalis subsp. lactis | Bla019 | 5-400B | |
| Bifidobacterium animalis subsp. animalis | BA023 | 5-500B | |
| Bifidobacterium breve | BB033 | 5-200B | |
| Bifidobacterium longum subsp. longum | BL5b | 5-100B | |
| Bidobacterium longum subsp. infantis | BI08 | 5-50B | |
| Bifidobacterium bifidum | BMC31 | 5-200B | |
| Bifidobacterium Adolesentis | BAA021 | 5-100B | |
| Streptococcus salivarius subsp. thermophilus | ST076 | 5-300B | |
| Pediococcus Pentosaceus | PP016 | 5-300B | |
| Pediococcus acidolactici | PAL31 | 5-400B | |
| Leuconostoc mesenteroides subsp. mesenteroides | LCM020 | 5-200B | |
| Weizmannia coagulans | BC01 | 5-100B | |
| Akkermansia muciniphila | AMP018 | 5-200B | |
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