The vaginal and multiple funtion probiotics L. crispatus

• Vaginal health

L. crispatus can help protect against bacterial vaginosis, vulvovaginal candidiasis, and urinary tract infections. It can also help prevent pregnancy complications, such as premature birth. 

• Vaginal microbiota

L. crispatus can help restore vaginal microbiota after intrauterine surgery and inhibit endometrial fibrosis. It can also help promote the stability of normal vaginal microbiota. 

• Sexually transmitted diseases

L. crispatus can help reduce the risk of sexually transmitted diseases, such as HIV. 

• Immune response

L. crispatus can modulate the immune response. 

• Antibiotic sensitivity

L. crispatus can increase antibiotic sensitivity. 

• Pathogenic bacteria

L. crispatus can inhibit the colonization and survival of reproductive tract pathogens. It can also inhibit various genital tract pathogenic bacteria, such as Gardnerella vaginalis. 

• Fungi

L. crispatus can inhibit various genital tract fungi, such as Candida albicans. 

• Bactericidal substances

L. crispatus produces bactericidal substances, such as lactic acid and hydrogen peroxide. 

Lactobacillus crispatus (L. crispatus) is a type of probiotic ("good" bacteria) found naturally in the human body. It's also found in fermented foods.

"Good" bacteria such as L. crispatus might help the body break down food, absorb nutrients, and fight off "bad" organisms that might cause diseases. These bacteria are sometimes added to fermented foods like yogurt and also found in dietary supplements.

People use L. crispatus for overgrowth of bacteria in the vagina (bacterial vaginosis), infertility, urinary tract infections (UTIs), and many other conditions, but there is no good scientific evidence to support most of these uses.

Don't confuse L. crispatus with other Probiotics, or with fermented food products such as fermented milk, kefir, or yogurt. These are not the same. Also note that the Lactobacillus genus was split up into 25 different genera in April 2020. Many species were reclassified at this time, but L. crispatus remains in the Lactobacillus genus. Its name did not change.

Dosing

For adults, L. crispatus has been taken by mouth and also applied to the vagina in a capsule or suppository in varying doses. Speak with a healthcare provider to find out what type of product and dose might be best for a specific condition.

It's been used safely in doses of up to 2 billion CFUs daily for 5 days or twice weekly for 10 weeks. Pregnancy and breast-feeding: There isn't enough reliable information to know if L. crispatus is safe to use when pregnant or breast-feeding. But there are no reasons to expect safety concerns when used appropriately.

Storage

The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long- term storage, the vapor phase of a liquid nitrogen freezer is recommended.

 Lactobacilli MRS agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent

Incubation:

Temperature: 37°C

Atmosphere: Aerobic (with or without 5% CO2) or anaerobic

Propagation:

1. Keep vial frozen until ready for use, then thaw.

2. Transfer the entire thawed aliquot into a single tube of broth.

 3. Use several drops of the suspension to inoculate an agar slant and/or plate.

4. Incubate the tube, slant and/or plate at 37°C for 1 to 2 days

More;